Sorbent Polymer Extraction and Remediation System (SPEARS)

PCBs have been shown to cause cancer in animals and to have other adverse effects on immune, reproductive, nervous, and endocrine systems. Although the production of PCBs in the United States has been banned since the late 1970s, many surfaces are still coated with PCB-laden paints. The presence of PCBs in paints adds complexity and expense for disposal. Some treatment methods (e.g., use of solvents, physical removal via scraping) are capable of removing PCBs from surfaces, but these technologies create a new waste stream that must be treated. Other methods, like incineration, can destroy the PCBs but destroy the painted structure as well, preventing reuse. To address limitations with traditional abatement methods for PCBs in paints, researchers at NASAs Kennedy Space Center (KSC) and the University of Central Florida have developed the Activated Metal Treatment System (AMTS) for Paints. This innovative technology consists of a solvent solution (e.g., ethanol, d-limonene) that contains an activated zero-valent metal. AMTS is first applied to the painted surface either using spray-on techniques or wipe-on techniques. The solution then extracts the PCBs from the paint. The extracted PCBs react with the microscale activated metal and are degraded into benign by-products. This technology can be applied without removing the paint or dismantling the painted structure. In addition, the surface can be reused following treatment.

There is a significant need for an inexpensive biological approach to recover specific, targeted metals and other target materials in e-waste or other aqueous solutions that requires minimal input of resources, including energy. This invention is a method of removing or adsorbing a target substance or material, for example, a metal, non-metal toxin, dye, or small molecule drug, from solution, by functionalizing a substrate with a peptide configured to selectively bind to the target substance or material and to bind to the substrate. The substrate is fungal mycelium, and the naturally-occurring or bioengineered peptide is called a target-binding domain, which is chemically bonded to a selected solid substrate. The target chemical species binds to the target-binding domain and is removed from solution. The target can be any chemical species dissolved or suspended in the solution. Capture of the target by the substrate can isolate and allow removal of the target substance from solution, or for utilization in water filtration, or recovery of targeted chemical species from solution, particularly aqueous solution applications. The peptides used include (i) fusion peptides and/or proteins containing metal-binding domain sequence and optionally containing substrate-binding domain sequence; (ii) fusion peptides/proteins containing a metal-binding domain and a chitin-binding domain; and (iii) nucleic acids encoding fusion peptides and/or proteins containing metal-binding domain sequence. The technology enables simple scale up to a level that could be successfully implemented in an environment with limited resources, such as on a space mission or on earth in developing countries with poor access to clean water.

Plant uptake of TCE from contaminated groundwater is a well-known phenomenon. During the photosynthesis process, plants metabolize the TCE into a byproduct called trichloroacetic acid (TCAA). TCAA has been found to be a good indicator (or surrogate) molecule for the presence of TCE because it is more stable than TCE in plants. The hyperspectral estimator is being designed to detect TCAA. The method uses a white light that is directed at the surface of a plant's leaf. The interaction between the light and the leaf produces spectral signatures that are captured using a detector. A processor that will be coupled to the detector will compare these signatures to a library/database of signatures known to be indicators of the presence of TCAA (and thus TCE). The figure below on the left shows hyperspectral images captured using the method for leaves dosed with TCE over various time periods. These images are examples of response signatures that will eventually be built into the device's reference library/database. Proof-of-concept testing has shown that the hyperspectral estimator is capable of estimating the presence/absence of TCE in plant leaves with an accuracy of 80%. Efforts are now underway to further improve the accuracy of this method and to prototype the technology. The figure below on the right shows a diagram of the planned device.

Environmental Control and Life Support Systems (ECLSS) used for extended space missions must recover and process wastewater to provide potable water for crew consumption and oxygen generation. Exploration mission spacecraft will have a smaller crew than the ISS, meaning demands would typically be less than what full-featured commercial TOC analyzers are designed to provide. Current analyzer technology also has limitations and uncertainties for spaceflight integration, such as part traceability, reliability, material properties for flammability or off-gassing, software and interface that are inconsis-tent with spaceflight needs, human factors, and structural reliability. The MiniTOCA provides a compact solution to the performance demands of onboard water quality analysis for crewed exploration missions through a unique core technology process that facilitates the detection of trace organic compounds in a water sample. It utilizes an ultra-violet oxidation method to activate the dissolved oxygen in the water which results in oxidation of the organic chemicals into carbon dioxide. The carbon dioxide is then measured by a Miniature Tunable Laser Spectrometer (MTLS) by sweeping the carbon dioxide out of the water in a gas / liquid separator using nitrogen gas. This novel process allows for small system sample volumes, small overall size/mass, zero consumables, low average power con-sumption (less than 60W), projected long-life (~10 years), and reliable analytical performance – all addressing critical performance gaps within the current TOC analyzer industry. Lab and environmental testing demonstrated that the MiniTOCA’s architecture is both feasible and is excellent in performance. Potential commercial applications for the MiniTOCA include, but are not limited to, ultra-pure water (UPW) systems; remote, mobile, and distributed environmental water quality monitoring; and specialized industrial process control. Technologies comprising the device lend themselves to miniaturization and are forward leaning in exploration applications. The MiniTOCA is scheduled to be flown and imple-mented aboard the ISS in late 2025.

Because resupply of commodities for long duration space missions would be prohibitively expensive and could take an extensive length of time to reach habitats in orbit around or on other planetary bodies, it is critical that astronauts have the ability to recycle and reuse local waste streams to provide resources such as clean water, fuel, and nutrients for growing plants. Scientists at Kennedy Space Center and the University of South Florida have developed a technology that addresses this critical mission need. The modular system design incorporates all wastewater streams and some food waste including urine water, hygiene water, humidity condensate, Sabatier water, fecal waste, laundry water, and organic food waste. These sources are fed simultaneously into the system, and a function-driven, sequential purification process occurs. The primary processes include carbon conversion, phase separation (solid/liquid/gas), disinfection, nutrient/salts management, and salts balancing to generate a clean water stream. The heart of the closed-loop bio-regenerative system is an anaerobic membrane bioreactor (AnMBR), which takes raw wastewater streams and utilizes an anaerobic microbial consortium to carry out the breakdown of the organic matter. An ultrafiltration membrane captures and destroys pathogenic bacteria and viruses. The AnMBR system generates a clean water stream containing fertilizer constituents which can be used to cultivate either microalgae (for food, pharma/nutraceuticals, fuel or bioplastics) in photobioreactors or crops in hydroponic systems. The system also generates methane and hydrogen gas which can be used for fuel (or conversion to bioplastics), and CO2 which can be used to support plant growth.