Soil Remediation With Plant-Fungal Combinations

The technology builds on the existing notion that establishment of trees in contaminated soils can be enhanced through the use of ectomycorrhizal (EM) fungi. EM fungi impart resistance to soil extremes such as high temperature, high acidity and heavy metal contamination. This process for soil remediation utilizes specific plant/fungal combinations that are specifically adapted to conditions created by phenolic application to soils, and abilities of ectomycorrhizal fungi to oxidize these compounds. This is done by taking advantage of the ability of native fungi to upregulate enzyme genes in response to changes in host physiological condition and hence enhance natural phenolic oxidation in soils by up to 5-fold. Ectomycorrhizal mediated remediation of phenolic- based contamination through use of specifically adapted ectomycorrhizal fungi and enzymes utilizes the findings that EM fungi in the genera Russula and Piloderma react with positive growth responses to phenolic-based soil contamination. The activities of enzymes that oxidize these compounds increase in activity by 5 fold when the host tree is partially defoliated, which in turn imparts an increase in phenolic oxidation in soils by a similar amount. Defoliation is done by pine needle removal, where 50% of the needles are removed. This process is performed each year on new growth to maintain defoliation.