Integrated Genomic and Proteomic Information Security Protocol

Cardiac muscle is myogenic and is capable of generating an action potential and depolarizing and repolarizing signals from within the muscle itself. An intrinsic conduction system (ICS), a group of specialized cardiac cells, passes an electrical signal throughout the heart. This technology is a method and associated system to identify a person based on the use of statistical parameters, peak amplitudes and/or time interval lengths and/or depolarization-repolarization vector angles and/or depolarization-repolarization vector lengths for PQRST electrical signals associated with heart waves. The statistical parameters, estimated to be at least 192, serve as biometric indicia to authenticate or to decline to authenticate an asserted identity of a candidate person. There are three on-line modes of operation enrollment, verification, and identification as well as two off-line modes statistics and settings. In enrollment the raw electrocardiography (ECG) signal is processed and the results in the form of parameters are serialized and saved. Verification and Identification procedures use the feature parameters for recognition (classification) of subjects based on the same kind of parameters (features) of heartbeats extracted from the ECG signal of a person to be verified or identified.

JSC's technology provides hazard-free, microgravity-compatible hardware for DNA/RNA isolation. It also allows PCR analysis to be used outside the lab in environments where pipetting is difficult and/or where hazardous chemicals must be confined to an enclosed container, such as military settings and remote clinical operations. This self-contained device for isolating DNA/RNA, proteins, and cells is a component system that includes syringes and pistons, membranes of different capacities, reagents, four-way valves, and small pumps. The pre-filled reagents are the same as those used in conventional PCR laboratory isolation analysis. The DNA and RNA isolation kits are novel and process small sample amounts using a self-enclosed and pipette-free technique. Multiple kits can be stacked to allow several samples to be processed simultaneously. The system can be used in conjunction with existing analysis modules, such as commercially available DNA instruments. The process can be fully automated and programmed and can potentially be applied to other biological processes. The JSC innovation will permit the extension of laboratory isolation protocols to many applications. This NASA Technology is available for your company to license and develop into a commercial product. NASA does not manufacture products for commercial sale.

This innovation is an autonomic method capable of transmitting a neutralizing data signal to counteract a potentially harmful signal. This otoacoustic component of an autonomic unit can render a potentially harmful incoming signal inert. For selfmanaging systems, the technology can offer a selfdefense capability that brings new levels of automation and dependability to systems.

Gene expression analysis measures the activity of genes and reveals valuable information about the internal states of living cells. NASA Ames has developed a novel system for conducting quantitative, real-time gene expression analysis that not only significantly improves the sample preparation procedures and provides a quick, cost-effective solution needed in a laboratory, but also minimizes the risk of RNA contamination and degradation. The invention, a novel assay methodology and suite of devices have been developed to isolate nucleic acids from Prokaryotic and Eukaryotic cells and prepare samples for Reverse Transcriptase - quantitative Polymerase Chain Reaction (RT-qPCR) analysis. The invention consists of a method to extract and purify nucleic acids from biospecimen that does not require organics or toxic chemicals, and provides containment for the liquids. The assay employs an aqueous-based non-alcohol method that yields robust RNA quality; and PCR reaction tubes that are pre-loaded with stabilized lyophilized reagents designed to perform RT-qPCR analysis - all in all enabling rapid, cost effective, and portable manual operation in the laboratory or remote field environments. This innovative technology greatly reduces preparation time to less than 10% of the time it would take using standard techniques. This technology could be used with any commercial quantitative Polymerase Chain Reaction (qPCR) machine.

In this method an autonomic entity manages a system through the generation of one or more stay alive signals by a hierarchical evolvable synthetic neural system. The generated signal is based on the current functioning status and operating state of the system and dictates whether the system will stay alive, initiate self-destruction, or initiate sleep mode. This method provides a solution to the long standing need for a synthetic autonomous entity capable of adapting itself to changing external environments and ceasing its own operation upon the occurrence of a predetermined condition deemed harmful.