In-Situ Resource Utilization (ISRU): Methylotrophic Microorganisms Expressing Soluble Methane Monooxygenase Proteins
health medicine and biotechnology
In-Situ Resource Utilization (ISRU): Methylotrophic Microorganisms Expressing Soluble Methane Monooxygenase Proteins (TOP2-283)
Methane Metabolism by Yeast
Overview
Long duration missions to planetary bodies and deep space will require new technological developments that support human habitation in transit and on distant bodies. Microorganisms are unique from the standpoint that they can be employed as self-replicating bio-factories to produce both native and engineered mission relevant bio-products. ISRU using In-Space Manufacturing (ISM) to generate mission relevant products could be logical for many applications. Products that consist of a significant amount of carbon (e.g. fuels, and foods etc.) could potentially be derived from single-carbon molecules available on long duration missions. NASA Ames has developed a novel patent-pending technology for an in-space bio-manufacturing of mission relevant bio-products using methane as the sole carbon substrate.
The Technology
Microorganisms are unique from the standpoint that they can be employed as self-replicating bio-factories to produce both native and engineered mission relevant bio-products. Methane (CH4) usage in In-Space Manufacturing (ISM) platforms has been discussed previously for human exploration and has been proposed to be used in physicochemical systems as a propulsion fuel, supply gas, and in fuel cells. Carbon Dioxide (CO2) is abundant on Mars and manned spacecraft. On the International Space Station (ISS), NASA reacts excess CO2 with Hydrogen (H2) to generate CH4 and Water (H2O) using the Sabatier System (Figure 1). The resulting water is recovered in the ISS, but the methane is vented to space. Recapturing this methane and using it for microbial manufacturing could provide a unique approach in development of in-space bio-manufacturing. Thus, there is a capability need for systems that convert methane into valuable materials. Methane (CH4) is a potential carbon substrate for methanotrophic microorganisms which are able to metabolize CH4 into biomass. The innovative technology from NASA Ames Research Center ports Soluble Methane Monooxygenase (sMMO) to Pichia, that is, it moves the methane metabolism into a robust microbial factory (Pichia pastoris) (Figure 2). The yeast Pichia pastoris is a refined microbial factory that is used widely by industry because it efficiently secretes products. Pichia could produce a variety of useful products in space. Pichia does not consume methane but robustly consumes methanol, which is one enzymatic step removed from methane. This novel innovation engineers Pichia to consume methane thereby creating a powerful methane-consuming microbial factory and utilizing methane in a robust and flexible synthetic biology platform.
Benefits
- Robust and cost-effective
- Microorganisms are unique from the standpoint that they can be employed as self-replicating bio-factories to produce both native and engineered mission relevant bio-products
- Carbon sources for microbial bio-manufacturing could be made from readily available carbon dioxide and methane
- Recapturing the lost methane in space and using it for microbial manufacturing could provide a unique approach in development of in-space bio-manufacturing
- Coupling an existing flight-approved technology and its waste stream to new systems such as bio-manufacturing
- In addition to Sabatier, methane could also be produced from other physico-chemical and non-physico-chemical technologies
- Several methanotrophic generated bio-products that could have mission relevance and unique capabilities in habitat construction, astronaut nutrition, and biomedical applications
- Scalable, programmable and regenerable
Applications
- Space industry - Habitat construction, astronaut nutrition, and biomedical applications
- Terrestrial methane-based microbial bio-manufacturing
- Biotechnology industry
Technology Details
health medicine and biotechnology
TOP2-283
ARC-17910-1
|
Tags:
|
Similar Results
Carbonated Cement for Production of Concrete with Improved Properties
The NASA cement innovation describes a method to make solid carbon material from CO2 captured during the cement-making process, and for using that carbon material in the mixture to improve cement properties. Doing so provides a direct use for the captured CO2, eliminating any CO2 storage/disposal issues and providing an improved cement product.
The innovation employs a chemical reaction, known as the Bosch process, which uses hydrogen gas and catalysis to reduce the CO2 to solid carbon and water. Cement manufacturing is uniquely suited to the use of the Bosch process. Cement manufacturing requires high temperatures, and harnessing this excess heat limits the total energy required to maintain a Bosch process at a cement plant. Also, cement contains iron, a metal shown to be an exceptional catalyst for the Bosch process. Thus, the cement product itself can be used as the catalyst for the reaction, also serving as a carbon sink. This eliminates any requirements for the storage or disposal of the waste carbon captured from CO2 emissions.
Test evaluations at the bench scale have provided encouraging indications of enhanced mechanical properties for the carbon-containing cement materials. In particular, the findings suggest that the carbon in the concrete might delay the environmental breakdown of concrete due to the blocking effect of the carbon on harmful ions (e.g., chlorine).
Low-Temperature Oxidation/
Reduction Catalysts
The low-temperature oxidation catalyst technology employs a novel catalyst formulation, termed platinized tin oxide (Pt/SnOx). The catalysts can be used on silica gel and cordierite catalyst supports, and the latest developments provide sprayable formulations for use on a range of support types and shapes. Originally developed for removal of CO, the catalyst has also proven effective for removal of formaldehyde and other lightweight hydrocarbons.
NASA researchers have also extended the capability to include reduction of NOx as well as developed advanced chemistries that stabilized the catalyst for automotive catalytic converters via the engineered addition of other functional components. These catalyst formulations operate at elevated temperatures and have performed above the EPA exhaust standards for well beyond 25,000 miles. In addition, the catalyst can be used in diesel engines because of its ability to operate over an increased temperature range.
For use as a gas sensor, the technology takes advantage of the exothermic nature of the catalytic reaction to detect formaldehyde, CO, or hydrocarbons, with the heat being produced proportional to the amount of analyte present.
Algae Photobioreactor Using Floating Enclosures With Semi-Permeable Membranes
The photobioreactors allow light to enter through their transparent upper surface and optimizes the efficiency of light utilization with a light-reflective lower surface inside. Deployed in the marine environment, the gradient between the freshwater inside the system and the saltwater outside drives forward osmosis. The water removed through semi-permeable (forward osmosis) membranes is cleaned as it is released into the marine environment. In addition, this process concentrates nutrients in the algae medium to stimulate growth, and concentrates the algae to facilitate harvesting. The harvested algae can be used to make biofuels, fertilizer, animal food, or other products. The photobioreactors are intended for use in naturally or artificially protected marine environments with small waves and gentle currents. The system can also be used in artificial brine pools and freshwater basins or reservoirs, however in freshwater the forward osmosis feature cannot be used.
Surface Densification Of Phenolic Impregnated Carbon Ablator (PICA)
The graded Thermal Protection System (TPS) offers a lower density than comparable state-of-the-art TPS systems operating at similar maximum heating conditions. This approach is straight forward in terms of processing and surface-treatment application and can be applied to machine PICA materials without having an effect on the final tolerance. The process results in increased usability and handling since standard uncoated PICA is relatively weak. Surface-densified PICA provides an approach for improvements in the robustness for the baseline CEV heat shield. A graded approach eliminates the need for joints and/or bonding agents between material plies. PICA surface densification offers robust mechanical protection against transit damage, handling damage, and in-flight object damage.
Modular Artificial-Gravity Orbital Refinery Spacecraft
Modular Artificial-Gravity Orbital Refinery Spacecraft is a solution for refining in-situ materials collected in space, such as from asteroids and Mars moons, as well as recycling spacecraft debris, while orbiting in micro-gravity conditions. The spacecraft is coupled with refining modules for refining and recycling different types of materials. It generates artificial gravity for operation in low-gravity environments. The spacecraft is comprised of rotating rings, each generating artificial gravity and angular momentum. When the rotating rings are combined on the spacecraft platform, however, they have a net near-zero angular momentum such that the spacecraft can change its attitude with minimal propellant or rotate at the rate of the object the spacecraft platform is attached to. The spacecraft platform can self-balance to accommodate different sized modules and modules with moving loads. The refined and recycled materials can be used to create products in-situ as well as products too large to launch from Earth, such as construction of orbiting space habitats, large spacecraft, solar-power stations, and observatories.
