Search

Gene expression analysis measures the activity of genes and reveals valuable information about the internal states of living cells. NASA Ames has developed a novel system for conducting quantitative, real-time gene expression analysis that not only significantly improves the sample preparation procedures and provides a quick, cost-effective solution needed in a laboratory, but also minimizes the risk of RNA contamination and degradation. The invention, a novel assay methodology and suite of devices have been developed to isolate nucleic acids from Prokaryotic and Eukaryotic cells and prepare samples for Reverse Transcriptase - quantitative Polymerase Chain Reaction (RT-qPCR) analysis. The invention consists of a method to extract and purify nucleic acids from biospecimen that does not require organics or toxic chemicals, and provides containment for the liquids. The assay employs an aqueous-based non-alcohol method that yields robust RNA quality; and PCR reaction tubes that are pre-loaded with stabilized lyophilized reagents designed to perform RT-qPCR analysis - all in all enabling rapid, cost effective, and portable manual operation in the laboratory or remote field environments. This innovative technology greatly reduces preparation time to less than 10% of the time it would take using standard techniques. This technology could be used with any commercial quantitative Polymerase Chain Reaction (qPCR) machine.

The parametric modeling system allows for the integrated design and optimization of aerospace vehicles by unifying physical and control subsystems within a single computational model. The system includes representations of the vehicle’s geometry, structural load, propulsion, energy storage, and GNC systems. The system performs sensitivity analysis on key performance metrics (e.g., fuel consumption, heat load, and mechanical forces) to determine how changes in design parameters affect overall performance. By incorporating real-world conditions, such as wind variations and sensor noise, the system allows for the use of real-time feedback to refine vehicle designs. The optimization process uses a gradient-based algorithm to iteratively adjust parameters so that constraints such as structural integrity, thermal protection, and fuel capacity are met. The system generates a Pareto front representing trade-offs between performance metrics that allow engineers to visualize optimal designs for different mission profiles, which enhances design accuracy while reducing the need for expensive physical testing.

The QCL source addresses the challenges of inefficiency, high power consumption, and bulky designs typically associated with existing solutions. It is fabricated with 80 to 100 alternating layers of semiconductor materials, each layer only a few microns thick. These layers create a cascade effect that amplifies terahertz-energy photon generation while consuming significantly less voltage. To mitigate the natural beam dissipation of QCLs, the source is integrated with a waveguide and thin optical antenna, reducing signal loss by 50%. Additionally, the waveguide employs a flared design with a diagonal feed horn, achieving high modal confinement and increasing beam coupling efficiency to 82%, compared to 37% in conventional setups. This compact design, smaller than a U.S. quarter, fits within payload constraints and enables high-powered terahertz beams for precise spectroscopic measurements. The terahertz transceiver enhances measurement precision by integrating two back-to-back hybrid couplers and Schottky diodes as detectors, providing a 35 dB dynamic range. Operating in the 2.0–3.2 THz frequency range, the transceiver is optimized for versatility across astrophysics, heliophysics, and planetary science applications. It seamlessly couples the QCL-generated signal onto the waveguide, ensuring stable and accurate spectroscopic data collection. This compact and energy-efficient transceiver delivers exceptional sensitivity, enabling it to analyze planetary materials, atmospheric components, and interstellar phenomena with unmatched resolution. With its compact, tunable design and high spectral resolution, the QCL source and transceiver represents a significant advancement for remote sensing and planetary surface characterization, offering a versatile solution for both NASA and commercial applications. The QCL system is at technology readiness level (TRL) 4 (component and/or breadboard validation in lab) and is available for patent licensing.

The technology provides miniaturized techniques for extracting trace amounts of organic molecules (lipids) from natural samples. It operates as an autonomous, miniaturized fluidic system, integrating lab techniques for lipid analysis while minimizing reagent volumes and concentrating organics for analysis, thereby increasing signal-to-noise ratios by orders of magnitude. The non-aqueous fluidic system described herein for astrobiological and life-detection missions (either in situ or returned sample) is configured to extract lipid organics from regolith using (1) a fluidic sample processor made of materials compatible with organic solvents and (2) a machine-learning system to select processing steps and parameters to maximize lipid yield. A critical gap is bridged by integrating technologies into a system that replicates analytical lab procedures autonomously on a spaceflight instrument scale with fidelity to original lab techniques. Automated fluidic devices combine controlled handling of liquids with sequential operations and parallelization of replicate processes. By designing such systems to closely interface with both sample-delivery and analytical measurement systems, laboratory analyses are automated. The technology adapts best practice laboratory methods for lipid analysis, overcoming analytical challenges like low organic abundance, interference of minerals/salts, and degradation of origin-diagnostic molecular structures. The extraction and concentration techniques from rock/soil samples can be applied to any biomarkers by changing the solvent, temperature, and agitation.

Although monochrome DIC is a favored technique for measuring tensile strain in soft goods materials, the approach has difficulties when materials are interwoven. As mentioned, traditional DIC software treats regions for analysis as a solid surface and incorrectly represents deformations much larger than those actually present during testing, thus yielding false results. Additionally, considerable investments of time and effort from a trained technician are necessary to parse out strains in each direction of biaxially woven material. This entails meticulously identifying areas of interest whenever an undulating directional weave is visible. The primary restraint layer of an inflatable space structure is comprised of orthogonally opposed, or interwoven straps. This DIC with RGB color filtering process presents a solution in providing more accurate and timely strain measurement of interwoven strap material by isolating a single strap direction for analysis. This approach leverages unique post processing capabilities developed at NASA to filter out red or blue photogrammetry patterns, allowing for tunable isolation of a particular color pattern and directional strap. When restraint layer material was analyzed using VIC-3D software, the addition of RGB color filtering demonstrated improved strain field accuracy when compared to monochrome DIC alone. Notably, the implementation of RGB filtering allows the selection of a larger area of interest that results in minimal influence from the orthogonally opposed straps. This streamlines the tensile strain analysis process, significantly reducing the time needed to analyze large bodies of interwoven material. “Digital Image Correlation with Color Filtering for Bi-Axially Strain Isolation”, has a technology readiness level (TRL) 6 (System/sub-system model or prototype demonstration in an operational environment), and it is now available for patent licensing.

The tool developed through this work merges information from the electric propulsion system design phase with diagnostic tools. Information from the failure mode and effect analysis (FMEA) from the system design phase is embedded within a Bayesian network (BN). Each node in the network can represent either a fault, failure mode, root cause or effect, and the causal relationships between different elements are described through the connecting edges. This novel approach can help Fault Detection and Isolation (FDI), producing a framework capable of isolating the cause of sub-system level fault and degradation. This system: Identifies and quantifies the effects of the identified hazards, the severity and probability of their effects, their root cause, and the likelihood of each cause; Uses a Bayesian framework for fault detection and isolation (FDI); Based on the FDI output, estimates health of the faulty component and predicts the remaining useful life (RUL) by also performing uncertainty quantification (UQ); Identifies potential electric powertrain hazards and performs a functional hazard analysis (FHA) for unmanned aerial vehicles (UAVs)/Urban Air Mobility (UAM) vehicles. Despite being developed for and demonstrated with an application to an electric UAV, the methodology is generalized and can be implemented in other domains, ranging from manufacturing facilities to various autonomous vehicles.

The one-piece multi-metallic composite overwrap thrust chamber assembly is centrally composed of an additively manufactured integral-channeled copper combustion chamber. The central chamber is being manufactured using a GRCop42 or GRCop84 copper-alloy additive manufacturing technology previously developed by NASA. A bimetallic joint (interface) is then built onto the nozzle end of the chamber using bimetallic additive manufacturing techniques. The result is a strong bond between the chamber and the interface with proper diffusion at the nozzle end of the copper-alloy. The bimetallic interface serves as the foundation of a freeform regen nozzle. A blown powder-based directed energy deposition process (DED) is used to build the regen nozzle with integral channels for coolant flow. The coolant circuits are closed with an integral manifold added using a radial cladding operation. To complete the TCA, the entire assembly including the combustion chamber and regen nozzle is wrapped with a composite overwrap capable of sustaining the required pressure and temperature loads.

This NASA invention utilizes a simple waveguide-based measurement system to determine the complex dielectric permittivity and magnetic permeability of arbitrary-shaped planetary rock samples. The system operates at L-band frequencies (~1 GHz) and can be extended to P- and S-bands for broader applications. The approach involves placing an arbitrarily-shaped sample inside an open-ended waveguide excited by a coaxial probe, measuring the scattering parameters, and extracting dielectric and magnetic properties through computational modeling and optimization techniques. A key aspect of this system is its ability to handle non-uniform and irregularly shaped rock samples, enabling the measurement of real-world planetary materials without requiring extensive sample preparation. The methodology includes calibration in an anechoic chamber, computational modeling, and iterative refinement of measured vs. simulated scattering parameters to extract the material properties. Future advancements will involve expanding measurements to different frequency bands, refining computational models using artificial intelligence, and automatically rotating samples within the waveguide to obtain multiple directional measurements (enhancing precision while reducing test time). This NASA innovation has been successfully applied to two Martian meteorite samples, yielding values of dielectric permittivity and permeability relevant for Mars radar applications. The system will further be leveraged to build an expansive database of the dielectric properties of planetary soils and rocks to improve radar-based mapping (e.g., subsurface mapping) missions. The invention could also be applied for the non-destructive screening of a variety of samples using radio waves, including biological samples for medical purposes, additive manufacturing feedstock or finished parts, and mining-related rock samples to test for impurities or resources of interest. This NASA invention is at technology readiness level (TRL) 5 (component and/or breadboard validation in relevant environment) and is available for patent licensing.

The technology is an adaptive data management and integration platform designed for disparate data sources. It is built to support multitenancy, manage data governance, handle heterogeneous data formats and advance data democratization using a suite of connected, independent microservices. Each service can be used within an integrated environment, or as a standalone product, with a dedicated set of functionalities, such as metadata management, data versioning, access control, data tagging, link management, and analytics, among others. The platform includes APIs to query, navigate and analyze complex interconnections between data assets. The invention provides the capability to capture and manage domain knowledge as a graph schema. The microservices architecture provides services for data tagging, managing data ownership, managing data relationships by dynamically associating data assets based on their content, metadata cataloging and handling, data discovery by searching data across domains ingested from various data sources, while tracking the data’s lineage and provenance, and product lifecycle that data assets belong to. Such data management can integrate heterogeneous datasets, facilitating cross-domain Metadata Management Services (MMS), identifying and limiting unconnected data sources and other fragmented data, as well as reducing redundant data sources.

To stain a specimen slide, one or more liquid reagents are injected via the dispenser into the slide staining device via a syringe port. The volume of a given reagent is determined by adjustable settings on the dispenser, so that when connected to the staining device, initiates a thin film over the slide. The dispensing device uses only a fraction of the reagents typically used in non-sealed environments. Medical grade polyvinyl alcohol sponges have been incorporated into the dispenser to provide additional fluid containment and retention during the staining procedure. Furthermore, the dispenser can recall excess reagent, minimizing reagent use until refill. The slide staining device is composed of an upper and lower section held together and aligned by use of Nd magnets. With the device open, a specimen slide is positioned upon a silicone gasket that sits within a recess in the lower section. When the device is closed, the silicone gasket in the upper section applies a seal to the slide forming a cavity that allows the slide to be exposed to reagents injected from the connected dispenser creating a stain through the use of capillary forces. Although originally designed for use in microgravity, the slide staining system also works in gravity environments. Numerous applications may exist for this technology, particularly in hematology and cellular biology. Other applications could be considered for academic research, veterinary field use, military, disaster stricken and remote environments or where fine control of fluid delivery, removal, and management is desired. The slide staining system is at technology readiness level (TRL) 8 (actual system completed and "flight qualified" through test and demonstration), and are now available to license. Please note that NASA does not manufacture products itself for commercial sale.